Rt-PCR

Real-time PCR (Polymerase Chain Reaction) is a widely used technique in molecular biology. This technique allows to quickly and precisely replicate a specific sequence of nucleic acids targeting a piece of DNA. A real-time PCR device is a device that monitors and measures this process in real time. It is used to directly track the amount of DNA generated in each cycle during the reproduction of DNA targets. It contains a special reagent that produces a fluorescent signal as the process of replication progresses. With the progression of the reaction, as the amount of target DNA increases, so does the fluorescent signal. Real-time PCR devices can quantitatively evaluate results by using a reference standard to determine how much of the target DNA is initially present. RT-PCR, which is used in many different fields due to its wide technical properties, is used in different fields such as DNA copy number measurement, separation of alleles, mutation detection, genotyping, drug treatment effectiveness and DNA damage measurements. Real-time PCR devices are used in medicine, microbiology, genetic research, food safety, environmental science and many other fields. In our laboratory, comparative gene quantification, gene expression analysis, miRNA expression analysis, non-coding RNA expression analysis and SNP genotyping services are offered to use with both SYBR Green and TaqMan methods.