What is toROMAN ™

The full name of the method is "Microfluidic Sperm Sorting Chips". It is used to obtain healthy sperm which required for in vitro fertilization, ICSI and intrauterine insemination treatments that applied to the patient is the same, except that sperm sorting is normally selected with the human eye among millions of sperm under the microscope. In this method it is selected by toROMAN ™

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In which treatments is toROMAN™ product used?

toROMAN™ can be used in traditional in vitro fertilization (IVF) and Intrauterine Sperm Injection (IUI) treatments. toROMAN™ is developed and manufactured specifically for cover all treatment methods for handle much more sample.

What are the differences between toROMAN™ and other microfluidic techniques?

• It can be checked with a microscope during the procedure.

• Uncomplicated manipulations, user-friendly.

• Can be used without mineral oil.

• Take approximately 1.0 mL of sperm sample.

• Simple incubation in 90 mm petri dish.

• Better results in DNA fragmentation and concentrations.

 

Where the "toROMAN ™" name come from?

Strength from the Past, Vision for the Future: toROMANTM

Our high-tech medical device, toROMANTM, takes its name from the Turkish word “toraman”, which means “strong and solidly built.” Historically, this word has symbolized power, durability, and reliability. Inspired by this traditional meaning, we have combined it with modern engineering to create a product that reflects both strength in its name and trust in its function.

toROMANTM is specifically designed to streamline the sperm preparation process in IVF treatments. With its advanced technological infrastructure and user-friendly interface, it enables sperm samples to be processed with greater precision, speed, and reliability. The device not only simplifies manual procedures but also provides healthcare professionals with the tools to enhance quality and increase success rates at every stage.

By fusing the strength of tradition with the potential of cutting-edge innovation, toROMANTM introduces a breakthrough solution in the field of reproductive health. This innovative device, which bridges the robust heritage of the past with the intelligent systems of the future, is developed to make IVF treatments more efficient, faster, and safer.

Performance Evaluation of "toROMAN™" Product.

In the first performance evaluation of the toROMAN™ product, a model with Density Gradient and the most well-known microfluidic sperm sorting chip was used in the same environment. In the evaluations, concentration, motility and DNA fragmentation index results were compared. Fertility rates, clinical pregnancy and birth rates will be evaluated through ongoing studies.

toROMAN™ Usage Protocol:

  1. First, incubate the collected semen sample at room temperature for 15–25 minutes to allow liquefaction.
  2. During this period, keep the toROMAN™ device at 37ºC until use.
  3. Using a 100–1000 µL pipette or a 1 mL syringe, load the sperm washing solution into the outlet area according to the volumes specified in the table below. After loading, wait for 10–15 seconds to ensure that all microchannels are completely filled.
  4. Using a 100–1000 µL micropipette equipped with the recommended tip or a 1 mL syringe, slowly dispense the semen sample volumes indicated in the table into the inlet ports. Allow the semen sample to drip freely. Do not touch the surface with the pipette tip.
Sorting Solution Volume Sample Volume for Each Inlet
750 µL 75 µL
1000 µL 100 µL
  1. Afterwards, the prepared toROMAN™ device is incubated for 2 hours at 37°C in a CO₂-free incubator with gentle handling.
  2. Following incubation, the preparation is examined under an inverted microscope, and the sperm cells reaching the full outlet area are evaluated.

7. Using a micropipette, approximately 750 µL of sorted sperm cells can be collected from the outlet area.

  1. The collected sperm cells are then transferred to the designated treatment areas.